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Technical Program

Paper Detail

Paper:	SS-11.2
Session:	Image Formation and Analysis for Molecular and Cellular Imaging
Time:	Friday, May 19, 14:20 - 14:40
Presentation:	Special Session Lecture
Topic:	Special Sessions: Image formation and analysis for molecular and cellular imaging
Title:	Quantum Dots Nano-Particles Full Field Imaging With Optical Sectioning and 3D Localization
Authors:	Frederic Chasles, Benoit Dubertret, CNRS, France; Claude Boccara, ESPCI, France
Abstract:	We present in this paper a technique for imaging fluorescent particles based on the axialmodulation of the objective’s focal plane position. This technique provides full field optical sectioning and can be used to localize the fluorophores in three dimensions. We describe the technique and apply it to image polymer beads filled with quantum dots immobilized in a gel. We show that full field optical sectioning is obtained and that the beads are localized with a precision of 10 nm in the transverse plane and 31 nm in the axial direction.